This is a list of characters who were antagonists during Day 2.
Joseph Wald cell
- Joseph Wald: American terrorist ringleader who planned to bomb CTU, killed himself.
- Eddie Grant: Hired by Wald to blow up CTU; killed by Jack during a shootout.
- Chris: Member of Eddie Grant's crew; killed by Jack during a shootout.
- Scott: Member of Eddie Grant's crew; killed by Jack during a shootout.
- Dave: Worked with Eddie Grant; beaten up and replaced by Jack.
- Frank: Member of Eddie Grant's crew; discovered the head of Marshall Goren
- Nina Myers: Sold schematics of CTU to Wald, held Jack hostage after she killed her informant, led CTU to Syed Ali.
Second Wave
- Syed Ali: Head of the Second Wave terrorists; killed by Jonathan Wallace to keep him quiet.
- Mamud Rasheed Faheen: Nina Myers' contact in Visalia; murdered by Nina with a broken gift card.
- Store clerk: One of the terrorists posing as a civilian at Mamud Rasheed Faheen's store.
- Jason Park: Tortured in the beginning of the day; revealed that a nuclear weapon would be used on American soil.
- Marie Warner: Daughter of Bob Warner and the sister of Kate. Funneled funds from her father's companies to support Ali. Later murdered her fianceé and equipped the nuclear bomb. Captured and incarcerated.
- Marko Khatami: Engineer working for Syed Ali. Shot by Basheer for deciding not to use the bomb.
- Basheer: Terrorist who shot Marko when he had doubts about killing so many people; Marko killed him before expiring.
- Omar: Drove the bomb van after Basheer and Marko killed each other. Pilot of the Cessna plane, captured and interrogated.
- Mohsen: Tortured Paul Koplin and Kate Warner; committed suicide during a CTU field operation.
- Mamud Rasheed Faheen: Nina Myers' contact in Visalia; murdered by Nina with a broken gift card.
Second Wave manipulation conspiracy
- Roger Stanton: Director of the NSA, allowed the nuclear weapon to come into the country but intended no loss of life.
- Sherry Palmer: Assisted Stanton and had limited contact with Peter Kingsley in order to ruin her ex-husband, President David Palmer.
- Bryce: Sherry Palmer's bodyguard.
- Ron Samuels: The colonel who controlled the Coral Snake soldiers.
- The Coral Snake unit: A paramilitary group which shadowed the nuclear weapon, waiting to take it out at the last possible second; shot down a plane full of federal agents including Jack Bauer.
- Senator Bruce Gluck: Supported and conspired with Stanton.
Oil consortium warmonger conspiracy
- Max: German oil consortium businessman; mastermind behind the day's events.
- Alexander Trepkos: Co-conspirator with Max and member of the oil consortium.
- Peter Kingsley: Businessman hired by Max against Trepkos's advice; killed by a CTU sniper.
- Ronnie Stark: Tortured Jack Bauer for proof of Cyprus' forgery; killed by Raymond O'Hara for stopping Jack's heart.
- Raymond O'Hara: Tortured Jack Bauer to replace Ronnie Stark. Killed by Jack Bauer.
- Trask: Mercenary working beneath Stark and O'Hara. Killed by Jack Bauer.
- Davis: Mercenary working beneath Stark and O'Hara. Killed by Jack Bauer.
- Eve: Fully involved in the conspiracy with Peter Kingsley until he found reason to kill her.
- Mae: an assistant to Peter Kingsley
- Scott: Kingsley's sniper.
- Jonathan Wallace: Coral Snake leader, working for Peter Kingsley; shot and killed by Kingsley's men.
- Wallace's accomplice: Worked with Wallace, attempted to kidnap Kate Warner.
- 10 hitmen, 6 thugs (unnamed)
- Peter Kingsley: Businessman hired by Max against Trepkos's advice; killed by a CTU sniper.
- Alex Hewitt: Computer hacker who designed the Cyprus recording; fell off a roof and died from his injuries.
- Mandy: Professional assassin hired by Max to assassinate David Palmer.
Racist rioters
- Marcus: Attacked Yusuf Auda because he was Middle Eastern; attempted to rob and murder Kate Warner.
- Rouse: An accomplice of Marcus.
- Cole: An accomplice of Marcus who stole evidence from Yusuf Auda.
- Racist riot leader: The spokesman for the massive mob that formed in Marietta, Georgia, who was interviewed by a FOX reporter.
Miscellaneous
- Gary Matheson: Beat his daughter, murdered his wife and a police officer, and attempted to kill Kim Bauer.
- Ramon Garcia: A civilian who panicked after the nuclear detonation who unintentionally killed Frank Davies and recklessly threatened the life of Kim Bauer.
- Marshall Goren: Witness against Wald, shot by Jack in the conference room.
- Jesper Isberg: Locked Lynne Kresge in a storage room, chased her, and kicked her off the stairwell nearly killing her.